human il 1β il 1f2 duoset elisa kit (R&D Systems)
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Human Il 1β Il 1f2 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+il+1%CE%B2+duoset+elisa+kit/Human+IL-1+beta%2FIL-1F2+DuoSet+ELISA/pm41948935-211-8-15
Average 96 stars, based on 560 article reviews
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Concentration Assay:Article Title: Interleukin-1β Secretion by Macrophages in Patients with Colorectal Cancer in Relation to the Clinicopathological Characteristics of the Disease. Article Snippet: .. The concentration of IL-1β in the culture supernatants was measured using an enzyme-linked immunosorbent assay (ELISA) with a commercial Article Title: Interleukin-1β Secretion by Macrophages in Patients with Colorectal Cancer in Relation to the Clinicopathological Characteristics of the Disease Article Snippet: .. The concentration of IL-1β in the culture supernatants was measured using an enzyme-linked immunosorbent assay (ELISA) with a commercial Article Title: Inhibition of NLRP3 by a CNS-penetrating indazole scaffold Article Snippet: PBMCs were purified from 10 ml of blood samples (drawn the day before in heparin tubes) by centrifugation on lymphocytes separation medium (Eurobio) at 800g for 20 min without brake, and plated at 10 6 cells/ml in RPMI-1640 GlutaMax-1 supplemented with 1X PS and 10% FBS (Gibco). .. Cells were primed with 10 ng/ml LPS for 3 h, and then treated with BAL compounds, MCC950, or vehicle only (DMSO, final concentration 0.05 %) for 15 min before addition of 5 μg/ml nigericin for 2 h or 100 μg/ml MSU (Invivogen) for 3 h. Cell-free media were analysed using Enzyme-linked Immunosorbent Assay:Article Title: Interleukin-1β Secretion by Macrophages in Patients with Colorectal Cancer in Relation to the Clinicopathological Characteristics of the Disease. Article Snippet: .. The concentration of IL-1β in the culture supernatants was measured using an enzyme-linked immunosorbent assay (ELISA) with a commercial Article Title: Alpinetin Suppresses Zika Virus-Induced Interleukin-1β Production and Secretion in Human Macrophages. Article Snippet: The developed color was detected with a plate reader (BioTek Instruments, Winooski, VT, USA) set for detection at 570 nm. .. IL-1β level in the supernatants of the control and treatment groups was evaluated by the Article Title: Palmitic acid reduces cytosolic potassium and induces IL-1β production in lipopolysaccharide-primed human macrophages Article Snippet: .. For Kv channel inhibition studies, cells were pre-treated for 30 min with Dimethyl sulfoxide (DMSO) (vehicle control), 5 μM 4-AP, or 50 mM TEA after LPS priming, followed by 100 μM PA-BSA treatment for 6 h. Culture supernatants were collected, and IL-1β levels were quantified using the Article Title: Interleukin-1β Secretion by Macrophages in Patients with Colorectal Cancer in Relation to the Clinicopathological Characteristics of the Disease Article Snippet: .. The concentration of IL-1β in the culture supernatants was measured using an enzyme-linked immunosorbent assay (ELISA) with a commercial Article Title: Monocyte NLRP3 inflammasome and interleukin-1β activation modulated by alpha-1 antitrypsin therapy in deficient individuals Article Snippet: C3d levels were detected in plasma using a Human complement C3 ELISA kit (Cusabio) according to manufacturer’s protocol. .. IL-1β and TNF- was detected in plasma and cell free supernatants using Article Title: A druggable redox switch on SHP1 controls macrophage inflammation Article Snippet: For RA and MS patients ELISA assays, monocytes were plated in U-shaped-bottom 96-well plates (Fisherbrand, # FB012932 ) at 0.8×10 6 /ml and treated with 20 μM SCA1, SCA9, SCA7 or SCA25 for 3 h, followed by LPS stimulation (100 ng/ml) for 6 h. Cells were then pelleted at 500 g for 5 min, and TNF and IL-6 cytokine levels in the supernatants were quantified using human TNF or IL-6 DuoSet ELISA kits (R&D Systems, #DY210 or #DY206) according to manufacturer’s protocols. .. For measurement of IL-1β levels, monocytes were additionally treated with ATP (5 mM) for 45 min following LPS stimulation, pelleted at 500 g for 5 min, and supernatant IL-1β levels assayed using Article Title: Inhibition of NLRP3 by a CNS-penetrating indazole scaffold Article Snippet: PBMCs were purified from 10 ml of blood samples (drawn the day before in heparin tubes) by centrifugation on lymphocytes separation medium (Eurobio) at 800g for 20 min without brake, and plated at 10 6 cells/ml in RPMI-1640 GlutaMax-1 supplemented with 1X PS and 10% FBS (Gibco). .. Cells were primed with 10 ng/ml LPS for 3 h, and then treated with BAL compounds, MCC950, or vehicle only (DMSO, final concentration 0.05 %) for 15 min before addition of 5 μg/ml nigericin for 2 h or 100 μg/ml MSU (Invivogen) for 3 h. Cell-free media were analysed using Article Title: Quantifying the immune response to a tissue-engineered porcine extracellular matrix. Article Snippet: Ryaan EL-Andari1 , Sabin J Bozso1, Jimmy JH Kang1, Nicholas M Fialka2, David Al-Adra3, Steven R Meyer1, Michael C Moon1, Darren H Freed1 , Jayan Nagendran1 & Jeevan Nagendran*,1 1Division of Cardiac Surgery, Department of Surgery, University of Alberta, Edmonton, AB, T6G 2B7, Canada 2Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, T6G 2R7, Canada 3Division of Transplantation, Department of Surgery, University of Wisconsin School of Medicine & Public Health, Madison, WI 53705, USA *Author for correspondence: Tel.. : +1 780 492 7605; jeevan@ualberta.ca Control:Article Title: Alpinetin Suppresses Zika Virus-Induced Interleukin-1β Production and Secretion in Human Macrophages. Article Snippet: The developed color was detected with a plate reader (BioTek Instruments, Winooski, VT, USA) set for detection at 570 nm. .. IL-1β level in the supernatants of the control and treatment groups was evaluated by the Article Title: Palmitic acid reduces cytosolic potassium and induces IL-1β production in lipopolysaccharide-primed human macrophages Article Snippet: .. For Kv channel inhibition studies, cells were pre-treated for 30 min with Dimethyl sulfoxide (DMSO) (vehicle control), 5 μM 4-AP, or 50 mM TEA after LPS priming, followed by 100 μM PA-BSA treatment for 6 h. Culture supernatants were collected, and IL-1β levels were quantified using the Inhibition:Article Title: Palmitic acid reduces cytosolic potassium and induces IL-1β production in lipopolysaccharide-primed human macrophages Article Snippet: .. For Kv channel inhibition studies, cells were pre-treated for 30 min with Dimethyl sulfoxide (DMSO) (vehicle control), 5 μM 4-AP, or 50 mM TEA after LPS priming, followed by 100 μM PA-BSA treatment for 6 h. Culture supernatants were collected, and IL-1β levels were quantified using the Clinical Proteomics:Article Title: Monocyte NLRP3 inflammasome and interleukin-1β activation modulated by alpha-1 antitrypsin therapy in deficient individuals Article Snippet: C3d levels were detected in plasma using a Human complement C3 ELISA kit (Cusabio) according to manufacturer’s protocol. .. IL-1β and TNF- was detected in plasma and cell free supernatants using Staining:Article Title: Quantifying the immune response to a tissue-engineered porcine extracellular matrix. Article Snippet: Ryaan EL-Andari1 , Sabin J Bozso1, Jimmy JH Kang1, Nicholas M Fialka2, David Al-Adra3, Steven R Meyer1, Michael C Moon1, Darren H Freed1 , Jayan Nagendran1 & Jeevan Nagendran*,1 1Division of Cardiac Surgery, Department of Surgery, University of Alberta, Edmonton, AB, T6G 2B7, Canada 2Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, T6G 2R7, Canada 3Division of Transplantation, Department of Surgery, University of Wisconsin School of Medicine & Public Health, Madison, WI 53705, USA *Author for correspondence: Tel.. : +1 780 492 7605; jeevan@ualberta.ca |
