Review



human il 1β il 1f2 duoset elisa kit  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    R&D Systems human il 1β il 1f2 duoset elisa kit
    Human Il 1β Il 1f2 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+1%CE%B2+duoset+elisa+kit/Human+IL-1+beta%2FIL-1F2+DuoSet+ELISA/pm41948935-211-8-15
    Average 96 stars, based on 560 article reviews
    human il 1β il 1f2 duoset elisa kit - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Concentration Assay:

    Article Title: Interleukin-1β Secretion by Macrophages in Patients with Colorectal Cancer in Relation to the Clinicopathological Characteristics of the Disease.
    Article Snippet: .. The concentration of IL-1β in the culture supernatants was measured using an enzyme-linked immunosorbent assay (ELISA) with a commercial Human IL-1β DuoSet ELISA kit (R&D Systems Inc., USA). ..

    Article Title: Interleukin-1β Secretion by Macrophages in Patients with Colorectal Cancer in Relation to the Clinicopathological Characteristics of the Disease
    Article Snippet: .. The concentration of IL-1β in the culture supernatants was measured using an enzyme-linked immunosorbent assay (ELISA) with a commercial Human IL-1β DuoSet ELISA kit (R&D Systems Inc., USA). ..

    Article Title: Inhibition of NLRP3 by a CNS-penetrating indazole scaffold
    Article Snippet: PBMCs were purified from 10 ml of blood samples (drawn the day before in heparin tubes) by centrifugation on lymphocytes separation medium (Eurobio) at 800g for 20 min without brake, and plated at 10 6 cells/ml in RPMI-1640 GlutaMax-1 supplemented with 1X PS and 10% FBS (Gibco). .. Cells were primed with 10 ng/ml LPS for 3 h, and then treated with BAL compounds, MCC950, or vehicle only (DMSO, final concentration 0.05 %) for 15 min before addition of 5 μg/ml nigericin for 2 h or 100 μg/ml MSU (Invivogen) for 3 h. Cell-free media were analysed using human IL-1β Duoset ELISA kit (RnD Systems) and ELISA using anti-IL-18 (D044-3, Biotechne) and anti-IL-18-biotin (D045-6, Biotechne) as capture and detection antibodies. .. Human induced-pluripotent stem cell-derived microglia (iCell Microglia; R1131, Fujifilm) were directly thawed in poly-L-lysine coated 96-well plates at 50,000 cells/well in iCell glial base medium supplemented with iCell microglia supplements A, B, and C as per manufacturer’s instructions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Interleukin-1β Secretion by Macrophages in Patients with Colorectal Cancer in Relation to the Clinicopathological Characteristics of the Disease.
    Article Snippet: .. The concentration of IL-1β in the culture supernatants was measured using an enzyme-linked immunosorbent assay (ELISA) with a commercial Human IL-1β DuoSet ELISA kit (R&D Systems Inc., USA). ..

    Article Title: Alpinetin Suppresses Zika Virus-Induced Interleukin-1β Production and Secretion in Human Macrophages.
    Article Snippet: The developed color was detected with a plate reader (BioTek Instruments, Winooski, VT, USA) set for detection at 570 nm. .. IL-1β level in the supernatants of the control and treatment groups was evaluated by the human IL-1β DuoSet ELISA Kit (Cat No. DY201) (R&D Systems, Inc., Minneapolis, MN, USA) following the specific protocol instructed by the manufacturer. ..

    Article Title: Palmitic acid reduces cytosolic potassium and induces IL-1β production in lipopolysaccharide-primed human macrophages
    Article Snippet: .. For Kv channel inhibition studies, cells were pre-treated for 30 min with Dimethyl sulfoxide (DMSO) (vehicle control), 5 μM 4-AP, or 50 mM TEA after LPS priming, followed by 100 μM PA-BSA treatment for 6 h. Culture supernatants were collected, and IL-1β levels were quantified using the Human IL-1β DuoSet ELISA kit (R&D system) according to the manufacturer’s instructions. ..

    Article Title: Interleukin-1β Secretion by Macrophages in Patients with Colorectal Cancer in Relation to the Clinicopathological Characteristics of the Disease
    Article Snippet: .. The concentration of IL-1β in the culture supernatants was measured using an enzyme-linked immunosorbent assay (ELISA) with a commercial Human IL-1β DuoSet ELISA kit (R&D Systems Inc., USA). ..

    Article Title: Monocyte NLRP3 inflammasome and interleukin-1β activation modulated by alpha-1 antitrypsin therapy in deficient individuals
    Article Snippet: C3d levels were detected in plasma using a Human complement C3 ELISA kit (Cusabio) according to manufacturer’s protocol. .. IL-1β and TNF- was detected in plasma and cell free supernatants using Human IL-1β DuoSet ELISA kit (R&D Systems) or Human TNF- DuoSet ELISA kit (R&D Systems) according to manufacturer’s protocol. .. HC and AATD monocytes were cell sorted using the EasySep™ Human CD14 Positive Selection Kit II (Stemcell™ Technologies) according to manufacturer’s instructions.

    Article Title: A druggable redox switch on SHP1 controls macrophage inflammation
    Article Snippet: For RA and MS patients ELISA assays, monocytes were plated in U-shaped-bottom 96-well plates (Fisherbrand, # FB012932 ) at 0.8×10 6 /ml and treated with 20 μM SCA1, SCA9, SCA7 or SCA25 for 3 h, followed by LPS stimulation (100 ng/ml) for 6 h. Cells were then pelleted at 500 g for 5 min, and TNF and IL-6 cytokine levels in the supernatants were quantified using human TNF or IL-6 DuoSet ELISA kits (R&D Systems, #DY210 or #DY206) according to manufacturer’s protocols. .. For measurement of IL-1β levels, monocytes were additionally treated with ATP (5 mM) for 45 min following LPS stimulation, pelleted at 500 g for 5 min, and supernatant IL-1β levels assayed using human IL-1β DuoSet ELISA kit (R&D Systems, #DY201). .. Absorbance values at 450 and 540 nm were determined with a microplate reader (BMG LABTECH FLUOstar Omega microplate reader), and wavelength corrected absorbances were used to plot standard curves from which cytokine levels from supernatant samples were calculated by intrapolation using a 4-parameter logistic (4PL) regression on GraphPad Prism 9.

    Article Title: Inhibition of NLRP3 by a CNS-penetrating indazole scaffold
    Article Snippet: PBMCs were purified from 10 ml of blood samples (drawn the day before in heparin tubes) by centrifugation on lymphocytes separation medium (Eurobio) at 800g for 20 min without brake, and plated at 10 6 cells/ml in RPMI-1640 GlutaMax-1 supplemented with 1X PS and 10% FBS (Gibco). .. Cells were primed with 10 ng/ml LPS for 3 h, and then treated with BAL compounds, MCC950, or vehicle only (DMSO, final concentration 0.05 %) for 15 min before addition of 5 μg/ml nigericin for 2 h or 100 μg/ml MSU (Invivogen) for 3 h. Cell-free media were analysed using human IL-1β Duoset ELISA kit (RnD Systems) and ELISA using anti-IL-18 (D044-3, Biotechne) and anti-IL-18-biotin (D045-6, Biotechne) as capture and detection antibodies. .. Human induced-pluripotent stem cell-derived microglia (iCell Microglia; R1131, Fujifilm) were directly thawed in poly-L-lysine coated 96-well plates at 50,000 cells/well in iCell glial base medium supplemented with iCell microglia supplements A, B, and C as per manufacturer’s instructions.

    Article Title: Quantifying the immune response to a tissue-engineered porcine extracellular matrix.
    Article Snippet: Ryaan EL-Andari1 , Sabin J Bozso1, Jimmy JH Kang1, Nicholas M Fialka2, David Al-Adra3, Steven R Meyer1, Michael C Moon1, Darren H Freed1 , Jayan Nagendran1 & Jeevan Nagendran*,1 1Division of Cardiac Surgery, Department of Surgery, University of Alberta, Edmonton, AB, T6G 2B7, Canada 2Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, T6G 2R7, Canada 3Division of Transplantation, Department of Surgery, University of Wisconsin School of Medicine & Public Health, Madison, WI 53705, USA *Author for correspondence: Tel.. : +1 780 492 7605; jeevan@ualberta.ca

    Control:

    Article Title: Alpinetin Suppresses Zika Virus-Induced Interleukin-1β Production and Secretion in Human Macrophages.
    Article Snippet: The developed color was detected with a plate reader (BioTek Instruments, Winooski, VT, USA) set for detection at 570 nm. .. IL-1β level in the supernatants of the control and treatment groups was evaluated by the human IL-1β DuoSet ELISA Kit (Cat No. DY201) (R&D Systems, Inc., Minneapolis, MN, USA) following the specific protocol instructed by the manufacturer. ..

    Article Title: Palmitic acid reduces cytosolic potassium and induces IL-1β production in lipopolysaccharide-primed human macrophages
    Article Snippet: .. For Kv channel inhibition studies, cells were pre-treated for 30 min with Dimethyl sulfoxide (DMSO) (vehicle control), 5 μM 4-AP, or 50 mM TEA after LPS priming, followed by 100 μM PA-BSA treatment for 6 h. Culture supernatants were collected, and IL-1β levels were quantified using the Human IL-1β DuoSet ELISA kit (R&D system) according to the manufacturer’s instructions. ..

    Inhibition:

    Article Title: Palmitic acid reduces cytosolic potassium and induces IL-1β production in lipopolysaccharide-primed human macrophages
    Article Snippet: .. For Kv channel inhibition studies, cells were pre-treated for 30 min with Dimethyl sulfoxide (DMSO) (vehicle control), 5 μM 4-AP, or 50 mM TEA after LPS priming, followed by 100 μM PA-BSA treatment for 6 h. Culture supernatants were collected, and IL-1β levels were quantified using the Human IL-1β DuoSet ELISA kit (R&D system) according to the manufacturer’s instructions. ..

    Clinical Proteomics:

    Article Title: Monocyte NLRP3 inflammasome and interleukin-1β activation modulated by alpha-1 antitrypsin therapy in deficient individuals
    Article Snippet: C3d levels were detected in plasma using a Human complement C3 ELISA kit (Cusabio) according to manufacturer’s protocol. .. IL-1β and TNF- was detected in plasma and cell free supernatants using Human IL-1β DuoSet ELISA kit (R&D Systems) or Human TNF- DuoSet ELISA kit (R&D Systems) according to manufacturer’s protocol. .. HC and AATD monocytes were cell sorted using the EasySep™ Human CD14 Positive Selection Kit II (Stemcell™ Technologies) according to manufacturer’s instructions.

    Staining:

    Article Title: Quantifying the immune response to a tissue-engineered porcine extracellular matrix.
    Article Snippet: Ryaan EL-Andari1 , Sabin J Bozso1, Jimmy JH Kang1, Nicholas M Fialka2, David Al-Adra3, Steven R Meyer1, Michael C Moon1, Darren H Freed1 , Jayan Nagendran1 & Jeevan Nagendran*,1 1Division of Cardiac Surgery, Department of Surgery, University of Alberta, Edmonton, AB, T6G 2B7, Canada 2Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, T6G 2R7, Canada 3Division of Transplantation, Department of Surgery, University of Wisconsin School of Medicine & Public Health, Madison, WI 53705, USA *Author for correspondence: Tel.. : +1 780 492 7605; jeevan@ualberta.ca



    Similar Products

    96
    R&D Systems human il 1β il 1f2 duoset elisa kit
    Human Il 1β Il 1f2 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+1%CE%B2+duoset+elisa+kit/Human+IL-1+beta%2FIL-1F2+DuoSet+ELISA/pm41948935-211-8-15
    Average 96 stars, based on 1 article reviews
    human il 1β il 1f2 duoset elisa kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    R&D Systems human il 1β duoset elisa kit
    a, LPS-induced (100 ng/ml) SHP1 Tyr536, Tyr564 and Ser591 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. b , LPS-induced (100 ng/ml) STAT3 Tyr705 and Ser727 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. c,e,f , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs pre-incubated 3 h with SCA1, SCA1-NC or the respective derivatives over a dose response (0.313–80 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 4). For measurement of pro-inflammatory <t>cytokine</t> <t>IL-1β</t> levels in cell supernatants, iBMDMs were pre-treated with LPS (100 ng/ml) for 3 h followed by treatment with DMSO or SCA9 at the indicated concentrations for 45 min and primed with adenosine triphosphate (ATP, 5 mM) for 45 min ( n = 3). Absolute cytokine production as a function of concentration is used to calculate IC 50 values. d , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs treated 3 h with SCA1 (20 μM) alone or 6 h with LPS (100 ng/ml) alone ( n = 3). g, Phagocytic activity of iBMDMs pre-treated 3 h with SCA1, SCA9, SCA7 or SCA25 (10 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 6). h, Relative fold changes in protein abundance between iBMDMs treated with SCA1 (50 μM) for 3 h followed by LPS (100 ng/ml, 15 min) versus LPS alone ( n = 3). Data are mean ± s.e.m. (in d,h ) or s.d. (in c,e-g ). P values calculated using one-way or two-way ANOVA for multiple comparisons or two-tailed Student’s t -tests for unpaired comparisons.
    Human Il 1β Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+1%CE%B2+duoset+elisa+kit/Human+IL-1+beta%2FIL-1F2+DuoSet+ELISA/pmc13070297-329-32-37
    Average 96 stars, based on 1 article reviews
    human il 1β duoset elisa kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    R&D Systems human il 1β elisa kit
    a, LPS-induced (100 ng/ml) SHP1 Tyr536, Tyr564 and Ser591 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. b , LPS-induced (100 ng/ml) STAT3 Tyr705 and Ser727 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. c,e,f , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs pre-incubated 3 h with SCA1, SCA1-NC or the respective derivatives over a dose response (0.313–80 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 4). For measurement of pro-inflammatory <t>cytokine</t> <t>IL-1β</t> levels in cell supernatants, iBMDMs were pre-treated with LPS (100 ng/ml) for 3 h followed by treatment with DMSO or SCA9 at the indicated concentrations for 45 min and primed with adenosine triphosphate (ATP, 5 mM) for 45 min ( n = 3). Absolute cytokine production as a function of concentration is used to calculate IC 50 values. d , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs treated 3 h with SCA1 (20 μM) alone or 6 h with LPS (100 ng/ml) alone ( n = 3). g, Phagocytic activity of iBMDMs pre-treated 3 h with SCA1, SCA9, SCA7 or SCA25 (10 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 6). h, Relative fold changes in protein abundance between iBMDMs treated with SCA1 (50 μM) for 3 h followed by LPS (100 ng/ml, 15 min) versus LPS alone ( n = 3). Data are mean ± s.e.m. (in d,h ) or s.d. (in c,e-g ). P values calculated using one-way or two-way ANOVA for multiple comparisons or two-tailed Student’s t -tests for unpaired comparisons.
    Human Il 1β Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+1%CE%B2+duoset+elisa+kit/Human+IL-1+beta%2FIL-1F2+DuoSet+ELISA/pm41587897-203-27-31
    Average 96 stars, based on 1 article reviews
    human il 1β elisa kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    R&D Systems elisa duoset kits against human il 1β
    a, LPS-induced (100 ng/ml) SHP1 Tyr536, Tyr564 and Ser591 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. b , LPS-induced (100 ng/ml) STAT3 Tyr705 and Ser727 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. c,e,f , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs pre-incubated 3 h with SCA1, SCA1-NC or the respective derivatives over a dose response (0.313–80 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 4). For measurement of pro-inflammatory <t>cytokine</t> <t>IL-1β</t> levels in cell supernatants, iBMDMs were pre-treated with LPS (100 ng/ml) for 3 h followed by treatment with DMSO or SCA9 at the indicated concentrations for 45 min and primed with adenosine triphosphate (ATP, 5 mM) for 45 min ( n = 3). Absolute cytokine production as a function of concentration is used to calculate IC 50 values. d , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs treated 3 h with SCA1 (20 μM) alone or 6 h with LPS (100 ng/ml) alone ( n = 3). g, Phagocytic activity of iBMDMs pre-treated 3 h with SCA1, SCA9, SCA7 or SCA25 (10 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 6). h, Relative fold changes in protein abundance between iBMDMs treated with SCA1 (50 μM) for 3 h followed by LPS (100 ng/ml, 15 min) versus LPS alone ( n = 3). Data are mean ± s.e.m. (in d,h ) or s.d. (in c,e-g ). P values calculated using one-way or two-way ANOVA for multiple comparisons or two-tailed Student’s t -tests for unpaired comparisons.
    Elisa Duoset Kits Against Human Il 1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+1%CE%B2+duoset+elisa+kit/Human+IL-6+DuoSet+ELISA/pmc12663425-80-0-13
    Average 96 stars, based on 1 article reviews
    elisa duoset kits against human il 1β - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    R&D Systems il 1β duoset elisa kit
    a, LPS-induced (100 ng/ml) SHP1 Tyr536, Tyr564 and Ser591 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. b , LPS-induced (100 ng/ml) STAT3 Tyr705 and Ser727 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. c,e,f , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs pre-incubated 3 h with SCA1, SCA1-NC or the respective derivatives over a dose response (0.313–80 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 4). For measurement of pro-inflammatory <t>cytokine</t> <t>IL-1β</t> levels in cell supernatants, iBMDMs were pre-treated with LPS (100 ng/ml) for 3 h followed by treatment with DMSO or SCA9 at the indicated concentrations for 45 min and primed with adenosine triphosphate (ATP, 5 mM) for 45 min ( n = 3). Absolute cytokine production as a function of concentration is used to calculate IC 50 values. d , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs treated 3 h with SCA1 (20 μM) alone or 6 h with LPS (100 ng/ml) alone ( n = 3). g, Phagocytic activity of iBMDMs pre-treated 3 h with SCA1, SCA9, SCA7 or SCA25 (10 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 6). h, Relative fold changes in protein abundance between iBMDMs treated with SCA1 (50 μM) for 3 h followed by LPS (100 ng/ml, 15 min) versus LPS alone ( n = 3). Data are mean ± s.e.m. (in d,h ) or s.d. (in c,e-g ). P values calculated using one-way or two-way ANOVA for multiple comparisons or two-tailed Student’s t -tests for unpaired comparisons.
    Il 1β Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+1%CE%B2+duoset+elisa+kit/Human+IL-1+beta%2FIL-1F2+DuoSet+ELISA/pmc12609157-222-10-14
    Average 96 stars, based on 1 article reviews
    il 1β duoset elisa kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    R&D Systems human il 1β dy201 elisa kits
    a, LPS-induced (100 ng/ml) SHP1 Tyr536, Tyr564 and Ser591 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. b , LPS-induced (100 ng/ml) STAT3 Tyr705 and Ser727 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. c,e,f , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs pre-incubated 3 h with SCA1, SCA1-NC or the respective derivatives over a dose response (0.313–80 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 4). For measurement of pro-inflammatory <t>cytokine</t> <t>IL-1β</t> levels in cell supernatants, iBMDMs were pre-treated with LPS (100 ng/ml) for 3 h followed by treatment with DMSO or SCA9 at the indicated concentrations for 45 min and primed with adenosine triphosphate (ATP, 5 mM) for 45 min ( n = 3). Absolute cytokine production as a function of concentration is used to calculate IC 50 values. d , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs treated 3 h with SCA1 (20 μM) alone or 6 h with LPS (100 ng/ml) alone ( n = 3). g, Phagocytic activity of iBMDMs pre-treated 3 h with SCA1, SCA9, SCA7 or SCA25 (10 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 6). h, Relative fold changes in protein abundance between iBMDMs treated with SCA1 (50 μM) for 3 h followed by LPS (100 ng/ml, 15 min) versus LPS alone ( n = 3). Data are mean ± s.e.m. (in d,h ) or s.d. (in c,e-g ). P values calculated using one-way or two-way ANOVA for multiple comparisons or two-tailed Student’s t -tests for unpaired comparisons.
    Human Il 1β Dy201 Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+1%CE%B2+duoset+elisa+kit/Human+IL-1+beta%2FIL-1F2+DuoSet+ELISA/pm41161541-80-5-13
    Average 96 stars, based on 1 article reviews
    human il 1β dy201 elisa kits - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    a, LPS-induced (100 ng/ml) SHP1 Tyr536, Tyr564 and Ser591 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. b , LPS-induced (100 ng/ml) STAT3 Tyr705 and Ser727 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. c,e,f , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs pre-incubated 3 h with SCA1, SCA1-NC or the respective derivatives over a dose response (0.313–80 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 4). For measurement of pro-inflammatory cytokine IL-1β levels in cell supernatants, iBMDMs were pre-treated with LPS (100 ng/ml) for 3 h followed by treatment with DMSO or SCA9 at the indicated concentrations for 45 min and primed with adenosine triphosphate (ATP, 5 mM) for 45 min ( n = 3). Absolute cytokine production as a function of concentration is used to calculate IC 50 values. d , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs treated 3 h with SCA1 (20 μM) alone or 6 h with LPS (100 ng/ml) alone ( n = 3). g, Phagocytic activity of iBMDMs pre-treated 3 h with SCA1, SCA9, SCA7 or SCA25 (10 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 6). h, Relative fold changes in protein abundance between iBMDMs treated with SCA1 (50 μM) for 3 h followed by LPS (100 ng/ml, 15 min) versus LPS alone ( n = 3). Data are mean ± s.e.m. (in d,h ) or s.d. (in c,e-g ). P values calculated using one-way or two-way ANOVA for multiple comparisons or two-tailed Student’s t -tests for unpaired comparisons.

    Journal: Nature chemical biology

    Article Title: A druggable redox switch on SHP1 controls macrophage inflammation

    doi: 10.1038/s41589-026-02163-8

    Figure Lengend Snippet: a, LPS-induced (100 ng/ml) SHP1 Tyr536, Tyr564 and Ser591 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. b , LPS-induced (100 ng/ml) STAT3 Tyr705 and Ser727 phosphorylation in iBMDMs over a time course of 2 h in iBMDMs pre-treated with DMSO or SCA1 (50 μM) for 3 h. Immunoblots shown are representative of three independent experiments. c,e,f , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs pre-incubated 3 h with SCA1, SCA1-NC or the respective derivatives over a dose response (0.313–80 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 4). For measurement of pro-inflammatory cytokine IL-1β levels in cell supernatants, iBMDMs were pre-treated with LPS (100 ng/ml) for 3 h followed by treatment with DMSO or SCA9 at the indicated concentrations for 45 min and primed with adenosine triphosphate (ATP, 5 mM) for 45 min ( n = 3). Absolute cytokine production as a function of concentration is used to calculate IC 50 values. d , Pro-inflammatory cytokine IL-6 and TNF levels in cell supernatants of iBMDMs treated 3 h with SCA1 (20 μM) alone or 6 h with LPS (100 ng/ml) alone ( n = 3). g, Phagocytic activity of iBMDMs pre-treated 3 h with SCA1, SCA9, SCA7 or SCA25 (10 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 6). h, Relative fold changes in protein abundance between iBMDMs treated with SCA1 (50 μM) for 3 h followed by LPS (100 ng/ml, 15 min) versus LPS alone ( n = 3). Data are mean ± s.e.m. (in d,h ) or s.d. (in c,e-g ). P values calculated using one-way or two-way ANOVA for multiple comparisons or two-tailed Student’s t -tests for unpaired comparisons.

    Article Snippet: For measurement of IL-1β levels, monocytes were additionally treated with ATP (5 mM) for 45 min following LPS stimulation, pelleted at 500 g for 5 min, and supernatant IL-1β levels assayed using human IL-1β DuoSet ELISA kit (R&D Systems, #DY201).

    Techniques: Phospho-proteomics, Western Blot, Incubation, Concentration Assay, Activity Assay, Quantitative Proteomics, Two Tailed Test

    a , Gating strategy for flow cytometry analysis of healthy donors or RA and MS patients PBMCs isolated monocytes using CD45, CD14 and CD16 staining ( n = 3 for each healthy and patient group). b , Percentage of CD45+CD14+CD16- monocyte population of interest ( n = 3 for each healthy and patient group). c , Pro-inflammatory cytokine TNF, IL-6 and IL-1β levels in cell supernatants of healthy donors and RA and MS patients monocytes pre-incubated 3 h with SCA1, SCA9, SCA7 and SCA25 (20 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 3 for each healthy and patient group). For measurement of pro-inflammatory cytokine IL-1β levels in cell supernatants, cells were additionally treated with adenosine triphosphate (ATP, 5 mM) for 45 min following LPS treatment. Data are mean ± s.e.m. P values calculated using two-tailed Student’s t -test for unpaired comparison or two-way ANOVA for multiple comparisons.

    Journal: Nature chemical biology

    Article Title: A druggable redox switch on SHP1 controls macrophage inflammation

    doi: 10.1038/s41589-026-02163-8

    Figure Lengend Snippet: a , Gating strategy for flow cytometry analysis of healthy donors or RA and MS patients PBMCs isolated monocytes using CD45, CD14 and CD16 staining ( n = 3 for each healthy and patient group). b , Percentage of CD45+CD14+CD16- monocyte population of interest ( n = 3 for each healthy and patient group). c , Pro-inflammatory cytokine TNF, IL-6 and IL-1β levels in cell supernatants of healthy donors and RA and MS patients monocytes pre-incubated 3 h with SCA1, SCA9, SCA7 and SCA25 (20 μM) followed by 6 h LPS stimulation (100 ng/ml) ( n = 3 for each healthy and patient group). For measurement of pro-inflammatory cytokine IL-1β levels in cell supernatants, cells were additionally treated with adenosine triphosphate (ATP, 5 mM) for 45 min following LPS treatment. Data are mean ± s.e.m. P values calculated using two-tailed Student’s t -test for unpaired comparison or two-way ANOVA for multiple comparisons.

    Article Snippet: For measurement of IL-1β levels, monocytes were additionally treated with ATP (5 mM) for 45 min following LPS stimulation, pelleted at 500 g for 5 min, and supernatant IL-1β levels assayed using human IL-1β DuoSet ELISA kit (R&D Systems, #DY201).

    Techniques: Flow Cytometry, Isolation, Staining, Incubation, Two Tailed Test, Comparison